Reporter

Part:BBa_J70570:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-04-12)


J04430 without the terminator BBa_J70570(AK)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 836


Design Notes

BsaXI site removed


Source

BsaXI site was removed from R0010 portion, pcr'd from J04430

J70569f and J70570f:

gtttcttcgaattcgcggccgcttctagaggattcattaatgcagctggcacgac

J70570r and J70571r:

gtttcttcctgcagcggccgctactagtattattatttgtatagttcatccatgccatg

References